An injury to the skin leads activation of a signaling cascade which produces nitric oxide as a signaling molecule, which in turn leads to angiogenesis proliferation and collagen deposition in the damaged wound area. Nitric oxide also plays a very crucial role in fighting the infection. Delay in healing process is essentially due to local infection (10⁵ colony counts/ g of tissue), Trauma, Foreign bodies, Malnutrition, Diabetes, Medication, Immunodeficiency. Unfortunately people who suffer from diabetes, or those who are elderly often do not produce enough nitric oxide naturally which can lead to poor wound healing. The Nitrogen oxides (primarily contains Nitric oxide, NO) releasing wound dressing is useful for the treatment of such chronic wounds. The dressing shows excellent poly-antimicrobial (antibacterial and antifungal) activity, including antibiofilm activity and is capable of sustained release of the active ingredient over long period of time.

ADVANTAGES

• The dressing is suitable for application on infected, non-healing wounds
• The dressing can be stored at ambient temperature and humidity conditions for two years, without loss of activity
• The dressing is relatively inexpensive, broad-spectrum in action and the drug active molecule does not lead to resistance development

APPLICATIONS

• The dressing is useful for treatment of chronic infected wounds and non- healing wounds (diabetic foot, pressure ulcer, venous stasis wound etc)
• The application of this dressing on wounds especially in diabetic patients can lead to decrease in the infection, increase in microvascularization and granulation thereby, leading to faster wound healing
• The dressing is also suitable for applications which require preparation of wound bed for acceptance of split skin graft based surgery

An injury to the skin leads activation of a signaling cascade which produces nitric oxide as a signaling molecule, which in turn leads to angiogenesis proliferation and collagen deposition in the damaged wound area. Nitric oxide also plays a very crucial role in fighting the infection. The delay in wound healing process is essentially due to local infection (10⁵ colony counts/ g of tissue), Trauma, Foreign bodies, Malnutrition, Diabetes, Medication, Immunodeficiency. Unfortunately people who suffer from diabetes, or those who are elderly often don?t produce enough nitric oxide naturally which can lead to poor wound healing. The nitrogen oxides releasing wound dressing is useful for the treatment of such chronic wounds. The dressing has shown excellent poly-antimicrobial (antibacterial and antifungal) activity, including antibiofilm activity and is capable of sustained release of the active ingredient over long period of time.

Working Principle of Dressing

The dressing works on the antimicrobial properties of Nitrogen oxides (NOx; the main component being nitric oxide). The dressing promotes angiogenesis, which helps in the graft acceptance to the wound bed. The NOx(g) released is be able to penetrate through the exopolysaccharide matrix of the biofilms in the wounds causing infection. Nitric oxide additionally has wound healing property thereby, aiding faster wound closure.

The dressing also contains collagen, a protein that gives the skin its tensile strength and plays a key role in wound healing. It attracts cells such as fibroblasts and keratinocytes to the wound, encourages angiogenesis and re-epithelialization. In addition, collagen provides a natural scaffold or substrate for new tissue growth.

Applications

• The dressing is useful for treatment of chronic infected wounds and non-healing wounds (diabetic foot, pressure ulcer, venous stasis wound etc)
• The application of this dressing on wounds especially in diabetic patients leads to control of biofilm, increased microvascularization and granulation thereby, leading to faster wound healing
• The dressing is also suitable for applications which requires preparation of wound bed for acceptance of split skin graft based surgery

Advantages

• The product is suitable for application on infected or non-healing wounds
• The dressing can be stored at ambient temperature and humidity conditions for up to two years, without loss of activity
• The dressing is easy to manufacture, relatively inexpensive, broad-spectrum in action and the active drug molecule does not lead to resistance development

Raw materials

• The raw materials are easily available in local market
  

Infrastructure

• A Chemical Laboratory having fume hood, Basic microbiology laboratory set-up, Access to Gamma chamber for irradiation, Hot air oven, Sonicating bath, UV source & Freeze-drier or any other alterative equipment are required

Manpower

• One trained person having degree in Chemistry & One person having degree in Microbiology with two semi-skilled workers would be adequate for production of nitrogen oxides releasing wound dressing