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Micropropagation of Banana

The Micropropagation protocol for banana developed at BARC includes tissue culture propagation using shoot tips on a defined nutrient media containing specific plant growth regulators. The steps include establishment of shoot tip cultures, induction of multiple shoots, elongation, rooting of shoots and hardening of rooted plantlets in the green house. The technique can be useful for rapid multiplication of banana cultivars. One of the main advantages is the production of disease free uniform age and size planting material which can be made available throughout the year.

Micropropagation of Banana

Banana is a globally important fruit crop with 97.5 million tones of annual production. In India, banana contributes 37% of the total fruit production and ranks second in importance next to mango. The state of Maharashtra ranks second with respect to the land under the cultivation but first in its production of banana with 60 T/ha per annum.

Propagation of Banana


Edible bananas do not produce seeds and are traditionally grown vegetatively through suckers (5 to 10 in number depending on the variety). Thus low rate of multiplication limits this method severely. In the recent years, tissue culture propagation of banana through shoot tip as well as floral aspices has been utilized to increase banana production. 
The process involves initiation of cultures from sterilized shoot tips obtained from the parent banana plant, shooting and rooting in the test tube, primary hardening in the laboratory, secondary hardening in the nursery and planting in the field.

Advantages of Tissue Cultured Banana

  • Disease free elite varieties
  • Rapid multiplication & Early harvesting
  • Uniform size and age of plants
  • High quality fruit bunches
  • Available throughout the year




Introduction 

Banana is a globally important fruit crop with 97.5 million tones of annual production. In India, banana contributes 37% of the total fruit production and ranks second in importance next to mango. The state of Maharashtra ranks second with respect to the land under the cultivation but first in its production of banana with 60 T/ha per anum.

Propagation of Banana


Edible bananas do not produce seeds and are traditionally grown vegetatively through suckers (5 to 10 in number depending on the variety). Thus low rate of multiplication limits this method severely. In the recent years, tissue culture propagation of banana through shoot tip as well as floral aspices has been utilized to increase banana production. 
The process involves initiation of cultures from sterilized shoot tips obtained from the parent banana plant, shooting and rooting in the test tube, primary hardening in the laboratory, secondary hardening in the nursery and plating in the field. 

Advantages of Tissue Cultured Banana

  • Disease free elite varieties
  • Rapid multiplication & Early harvesting 
  • Uniform size and age of plants
  • High quality fruit bunches
  • Available throughout the year

Requirements

  • Infrastructural requirements :
    A tissue culture laboratory, A green house or polyhouse facility and an agricultural plot for planting
  • Equipment Requirement :
    Standard glassware for media preparation and sterilization equipment like autoclave required for a tissue cultured laboratory
  • Manpower Requirement :
    Two technicians (preferably trained in a tissue culture laboratory) for laboratory work and other ancillary staff.
  • Capital Cost :
    Approximate cost for setting up of a medium size plant requires around 25 lakhs.
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General License Fee : ₹ 250,000.00 (Two Lakh Fifty Thousand)

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