-A A +A

This link shall take you to a page/website outside this website. For any query regarding the contents of the linked page/website, please contact the webmaster of the concerned website.

If you have already registered as a licensee then click on SIGN IN otherwise click on SIGN UP for register as a licensee.

Cold Plasma based Electroporator for Biological transformation (Electroporation)


  • NOTE: Facility for online Application for transfer of technology would be available soon...

Dielectric barrier discharge (DBD) mediated plasma is also known as cold plasma or atmospheric plasma. Recently, cold plasma has been shown to cause electroporation, i.e., the introduction of macromolecules into biological cells using electrical means. A portable DBD based cold plasma source along with innovative electrode and sample holding arrangement for achieving electro-transformation has been developed. Electroporation in the device is carried in a non-contact mode and it is economical compared to other conventional electroporation devices. 

Dielectric barrier discharge (DBD) mediated plasma is also known as cold plasma or atmospheric plasma. Recently, cold plasma has been shown to cause electroporation, i.e., the introduction of macromolecules into biological cells using electrical means. A portable DBD based cold plasma source along with innovative electrode and sample holding arrangement for achieving electro-transformation has been developed.

ADVANTAGES OF THE SYSTEM

  • This system is based on novel technology and is the first indigenous technology
  • The system is substantially cheaper compared to available commercial systems
  • The device operates on line voltage and does not require complex signal shaping electronics
  • Electroporation is carried in a non-contact mode and the sample preparation protocol is simpler
  • Electroporation does not require specialized sample holders

APPLICATIONS

  • Electroporation of bacterial cells with plasmids
  • Localized delivery of therapeutic agents or drugs
  • Transfection of mammalian cells to genetically modify cell lines

Dielectric barrier discharge (DBD) mediated plasma is also known as cold plasma or atmospheric plasma. Cold plasma exposure was demonstrated to kill microbes, primarily through membrane damage. Based on this property, a number of devices that are useful for surface sterilization have been developed. Recently, cold plasma has been shown to cause electroporation, i.e., the introduction of macromolecules into biological cells using electrical means. This process is also called electro-transformation. A portable DBD based cold plasma source along with innovative electrode and sample holding arrangement for achieving electro-transformation has been developed. This device facilitates electroporation of bacterial cells with high-voltage electrical signals. This technology is substantially cheaper compared to other imported systems. The instrument has been tested using different bacterial plasmid and different types of E. coli cells and was found to be reproducible.

MAIN COMPONENTS OF THE SYSTEM

  • High Voltage Power Supply, 6 kVrms
  • Cable / connector
  • Plasma Electrode Arrangement
  • Reaction chamber / Sample holder
  • Argon Gas cylinder (portable) with Gas flow monitor / meter

TECHNICAL SPECIFICATIONS

  1. The system operates on a 230 V AC (5 Amp) mains power supply
  2. The out-put of the power supply ranges from 100 to 7000 V, AC
  3. Dimensions (mm) of the power supply [WxHxD] : 250*150*300 ; Weight: ~7.5 kg
  4. Sample holder:

    a. Vertical displacement: 50 mm
    b. Holder base ~ 300 X 300 mm

  5. Argon cylinder (a portable cylinder is preferable)
  6. Important Parameters to be set:

    a. Voltage across the electrodes: 3500 to 6000 V
    b. Distance between the electrodes: 2 to 10 mm
    c. Gas flow-rate: control in the range of ~100 to 200 ml per minute. 

INFRASTRUCTURE

  • An electrical workshop having component assembly, SMD workbench & Instrument rack and high-voltage testing facility
  • 230 Volts, 5 Ampere, Single Phase Power supply
  • Access to a mechanical workshop having lathe and other machining equipment

MANPOWER

  • One person having Diploma in Electronics Engineering and one semi-skilled worker

SPACE

  • One work bench equivalent space (4?X6?) is required for assembly and testing

ADVANTAGES OF THE SYSTEM

  • This system is based on novel technology and is the first indigenous technology
  • The system is substantially cheaper compared to available commercial systems
  • The device operates on line voltage and does not require complex signal shaping electronics
  • Electroporation is carried in a non-contact mode and the sample preparation protocol is simpler
  • Electroporation does not require specialized sample holders

APPLICATIONS

  • Electroporation of bacterial cells with plasmids
  • Localized delivery of therapeutic agents or drugs
  • Transfection of mammalian cells to genetically modify cell lines
  • Apply for License
    NOTE:Facility for online Application for transfer of technology would be available soon...
  • SR NO. COUNTRY NAME PATENT NUMBER
    No Data Found.
    SR NO. NAME STANDARD NUMBER LOGO
    No Data Found.

    General License Fee : ₹ 49,000.00 (Forty Nine Thousand)

    General Royalty : 0%