Micropropagation of Pineapple
The Micropropagation protocol for pineapple developed at BARC includes tissue culture propagation using shoot tips as well as dormant axillary buds from pineapple crowns on a defined nutrient media containing specific plant growth regulators. The steps include sterilized shoot tips obtained from the parent pineapple plant, shooting and rooting in the test tube, primary hardening in the laboratory, secondary hardening in the nursery and plating in the field. One of the main advantages is the production of disease free uniform age and size planting material which can be made available throughout the year.
Pineapple (Ananas comosus L. Merr.) belongs to the family Bromeliaceae, and is one of the important commercial fruit crop propagated vegetatively. A good source of vitamins A and B, pineapple is fairly rich in vitamins C, calcium, magnesium, potassium and iron. It is also a source of bromelin, a digestive enzyme. At present pineapple is grown commercially in Assam, Meghalaya, Tripura, Mizoram, West Bengal, Kerala, Karnataka and Goa, and on a small scale in Gujarat, Maharashtra, Tamil Nadu, Andhra Pradesh, Orissa, Bihar and Uttar Pradesh. India ranked sixth with a share of about 8% of the world production of pineapples.
- Efficient and rapid multiplication protocol.
- Production of disease free planting material on a large scale.
- Production of plants throughout the year irrespective of the season.
- The technique can be employed for in vitro mutagenesis for inducing the genetic variability for the improvement of this fruit crop.
- Horticulture sector in general.
- The technology can be used by plant tissue culture companies.
- Farmers association and Krishi vigyan Kendra may use the technology for propagation and distribution of plants to end users.
PROPOGATION OF PINEAPPLE BY TISSUE CULTURE
Conventionally the average production is 4-5 propagules per year and it takes considerable time to produce enough planting material. Large-scale production of planting material can be achieved by using the plant tissue culture techniques.
Different stages of micropropagation of Pineapple are as depicted below-
ADVANTAGES OF TISSUE CULTURED PINEAPPLE
a. Infrastructural requirements
b. Equipment Requirement
c. Manpower Requirement
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